AP Chemistry
3.13 Beer-Lambert Law
A student uses clean cuvettes to prepare a calibration curve for an aqueous dye. The instrument is blanked with solvent, and the resulting calibration line has a positive slope and passes through the origin. For the spectrophotometer, \(A=-\log(T)\), where \(T=\dfrac{I}{I_0}\).
After collecting the calibration data, the student leaves fingerprint smudges on an optical face of the cuvette containing the unknown solution. The smudges are in the light-beam path, and the measured absorbance remains within the linear range of the calibration curve. Which choice correctly describes how the smudges affect the concentration calculated for the unknown?
After collecting the calibration data, the student leaves fingerprint smudges on an optical face of the cuvette containing the unknown solution. The smudges are in the light-beam path, and the measured absorbance remains within the linear range of the calibration curve. Which choice correctly describes how the smudges affect the concentration calculated for the unknown?
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